PUB HLTH 7119 MPH Dissertation

Question: A modified Folin–Ciocalteau method was carried out to mea-sure free and bound phenolics of the extracts (Fang, Zhang, Sun,& Sun, 2006), using gallic acid as a standard (0–360 mg/l). The freeor bound phenolic extracts (100 ll) were mixed with 2.5 ml of0.2 N Folin–Ciocalteau reagents, and then 2 ml of saturated sodiumcarbonate solution (75 g/l) were added. The absorbance of the mix-ture was determined at 765 nm using an UV-1800 Spectropho-tometer (Shimadzu, Canby, USA) after reaction for 2 h at roomtemperature in the dark. Results were expressed as mg gallic acidequivalents (GAE)/g dry basis, (db). Total phenolic content was calculated as the sum of free and bound. Answer: A Folin-Ciocalteau which is a modified method was employed to take the measurement of bound and free phenolics of the excerpts. This was carried by the use of gallic acid as a standard of 0-360mg/l. Folin-Ciocalteau of 2.5 ml of 0.2 N was mixed with extracts of bound or free phenolic reagents. After that about 2ml of sodium carbonate solution which is saturated and of the amount of 7.5 g/l were added. The mixture´s absorbance was obtained at 765nm by the means of UV-1800 spectrometer (Peter, 2013). This reaction was conducted for a period of two hours in a dark room and at room temperature. The results from the experiment were put as milligram gallic acid equivalent per dry basis db. The sum of phenolic content was obtained as the total of the bound and free. All the extracts were scrutinize and given in duplicate (James, 2010). Results Of Spectrometatic Values Data a Data b Raw Cica A 0.130 0.141 Raw Cica B 0.145 0.157 Raw Monarch A 0.198 0.119 Raw Monarch B 0.157 0.159 Raw tPM 2015 A 0.160 0.101 Raw tPM 2015 B 0.133 0.103 Raw tPM 2016A 0.142 0.189 Raw tPM 2016B 0.169 0.143 Raw tPM 2017A 0.115 0.158 Raw tPM 2017B 0.135 0.196 Raw 2nd Grade A 0.211 0.215 Raw 2nd Grade B 0.179 0.181 Freeze dried Cica A 0.164 0.165 Freeze dried Cica B 0.164 0.167 Freeze dried Monarch A 0.206 0.208 Freeze dried Monarch B 0.218 0.229 Freeze dried tPM 2015A 0.191 0.195 Freeze dried tPM 2015 B 0.187 0.190 Freeze dried tPM 2016 A 0.141 0.150 Freeze dried tPM 2016 B 0.110 0.119 Freeze dried tPM 2017A 0.185 0.190 Freeze dried tPM 2017 B 0.145 0.150 Freeze dried 2nd Grade A 0.129 0.130 Freeze dried 2nd Grade B 0.156 0.157 Standard Curve References: James, K. (2010). Health record keeping . London: Springer . Peter, K. (2013). Health Records . Hull: CRC.

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